Furthermore, induced pluripotent stem (iPS) cells can be generated by forced expression of specific transcription factors2.
Recent reports have shown that fibroblasts can be converted to neurons by forced expression of transcription factors.
Here we show that this 'missing-self' response can be prevented by forced expression of minimally polymorphic HLA-E molecules.
We previously reported rapid reprogramming of mouse embryonic fibroblasts (MEFs) into mature induced neuronal (iN) cells by forced expression of three transcription factors: ASCL1, MYT1L, and BRN2.
Li, P. et al. Accelerated generation of oligodendrocyte progenitor cells from human induced pluripotent stem cells by forced expression of Sox10 and Olig2.
Finally, we put our analysis in the perspective of recent attempts to directly reprogram cells to hepatocytes by forced expression of transcription factors.
Here, we show that human ESCs and iPSCs can be converted into functional iN cells with nearly 100% yield and purity in less than 2 weeks by forced expression of a single transcription factor.
Lineage reprogramming is an emerging field at the intersection of developmental and stem cell biology in which a somatic cell is stably reprogrammed into a distinct cell type by forced expression of lineage-determining factors.
Somatic cells can be reprogrammed to form embryonic stem cell-like induced pluripotent stem (iPS) cells by forced expression of a set of transcription factors1, indicating that terminally differentiated cells can be induced to undergo cell fate change.
Migration of CAR-T lymphocytes also improved by forced expression of CCR4.
(B) IPCs could also be induced from pancreatic non-β cells such as acinar cells and α-cells by forced expression of Ngn3 + Pdx1 + Mafa and Pax4, respectively[58].
