Coreceptor surface expression of CXCR4 and mutated CXCR4 was determined by fluorescent antibody staining using the conformation-dependent phycoerythrin labeled monoclonal antibodies (mAbs) 12G5 [ 38] (Pharmigen, Hamburg, Germany).
Apoptotic cells were identified by fluorescent double immunofluorescent staining using caspase-3 and terminal deoxyuridine nick-end labelling (TUNEL).
The distribution of actin cytoskeleton was observed by fluorescent staining.
Whitening pseudergates, identified by a whitening of the abdomen due to a gut purge before presoldier molting [21], and presoldiers within 24 hours after molting, were examined by retrograde staining using a fluorescent dextran.
The distinct phases of the cell cycle were classified by DNA staining using the fluorescent dye propidium iodide.
In vivo measurement of lipid content was performed by staining using the lipophilic fluorescent dye Nile red (Sigma) following van den Ecker et al. (2012) with the following modifications.
b Macrophages loaded with red fluorescent nanospheres infiltrating the brain metastases detected by immunofluorescent staining using anti-human CD68 antibodies labeled with APEX Pacific Blue fluorochrome.
Differentiation of BM-MSCs into hepatocytes was determined by immunofluorescence staining using albumin antibody and identification of BM-MSCs in hepatic fibrosis was determined by fluorescent microscopy using CELL STALKER-CSR dye staining.
