Sentence examples for by fluorescent microscopy using from inspiring English sources
Briefly, unfixed ovarian cancer cells or control cells in suspension or adherent to plastic were incubated with different concentrations of FITC-conjugated-C-CPE290-319 peptide for 30 to 60 minutes at 37°C and binding to cell surface expressed claudin-3 and claudin-4 was analyzed by fluorescent microscopy using a Nikon fluorescence microscope Eclipse E400.
Samples were examined directly by light microscopy (Olympus BX 45; Hamburg, Germany) or by fluorescent microscopy using an Olympus microscope with a 568-nm filter (CKX41).
After 2 days of transfection, GFP production was vizualized by fluorescent microscopy using a Zeiss Axioskop2 plus microscope.
Cells were visualised by fluorescent microscopy using the CellSens Olympus software.
Characteristic apoptotic morphological changes were assessed by fluorescent microscopy using bis-benzimide (Hoechst 33258) staining.
Characteristic apoptotic morphological changes were assessed by fluorescent microscopy using Hoechst 33258 staining.
The NPC1-YFP fluorescence produced by fibroblast skin cells in the biopsy can be detected by standard fluorescent microscopy using a ×20 objective and GFP filter set.
In the co-transfection studies COS7 cells were co-transfected with wild-type PLP EGFP and wild-type CK YFP, allowed to grow for 24 48 h, live-stained with Mitotracker Red 580, fixed and analysed by confocal fluorescent microscopy using the Leica TCS SP5 microscope (LASAF8.1.1), and images were processed as described above.
Following fixation and repeated washes, cover slips were incubated with the primary and secondary antibodies according to standard protocols, stained with propidium iodide 1 μg/ml and analyzed by confocal fluorescent microscopy using a Zeiss Axiovert 100 TV microscope and Zeiss software.
When indicated, FITC-conjugated Tat-S was used, and its entrance into cultured neurons was followed by in vivo fluorescent microscopy using an Eclipse TE2000-U Nikon microscope and a Hamamatsu digital camera C10600.
Binucleate (BN) cells were quantified by phase contrast and fluorescent microscopy using an inverted Leica DM IRB microscope; CellMask (plasma membrane dye/Invitrogen) and AlexaFluor 488 Phalloidin were used to demarcate the cytoplasm.
