Sentence examples for by fluorescent immunostaining using from inspiring English sources

Eve and Hb expression were detected by fluorescent immunostaining using anti-Eve (rabbit monoclonal) [37] and anti-Hb (mouse monoclonal 1G10) [54] antibodies for primary detection, and goat anti-rabbit IgG (H+L) AlexaFluor 488 or AlexaFluor 594 and goat anti-mouse IgG (H+L) AlexaFluor 488 conjugated secondary antibody (Molecular Probes) for secondary detection.

To test whether this predicted TF is expressed in Hcrt neurons, fluorescent ISH, using a probe for hsf1, and fluorescent immunostaining using an antibody against EGFP, were performed on the brain section of adult hcrt:EGFP zebrafish.

Leydig cells were identified by light microscopy and by fluorescent immunostaining for LH receptor expression using a previously described anti LH receptor antibody (Le Goffic et al., 2003) and a secondary rhodamine conjugated donkey anti-mouse IgG (dilution 1/50; Jackson Immunoresearch Laboratories Europe).

Furthermore, we detected the full-length peptide in cells by fluorescent immunostaining followed by SDS-PAGE.

The autoradiography images demonstrate laminar distribution of [18F]-9 binding in cortex, which correlates with the distribution of Aβ plaques detected by fluorescent immunostaining, and binding of [18F]-9 inhibitedted by excess cold ligand 5 (1 μM, C).

The autoradiography images demonstrate laminar distribution of [18F]-7B binding in cortex (bracket and arrows), which correlates with the distribution of Aβ plaques shown by fluorescent immunostaining, and binding of [18F]-7B inhibitedted by excess cold ligand (C).

These results were confirmed by fluorescent immunostaining.

Localization of these proteins in the ER was confirmed by fluorescent immunostaining of the immature caryopses.

SK-N-SH cells were shown to have overexpressed aggregates of TIAF1 and A β, as determined by fluorescent immunostaining.

To understand the mechanism of the developmental arrest phenotype, we checked the spindle organization of these Ago2-deficient oocytes by fluorescent immunostaining for tubulin.

Since we have observed lack of nuclear HES1 in PETs [ 8], protein expression in BON1 cells was investigated by fluorescent immunostaining.