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Blots were imaged by fluorescent imaging using the Odyssey infrared imaging system (LI-COR Biosciences; Millennium Science, Surrey Hills, Australia).
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FHA-labeled egg albumin and BSA were separated by SDS-PAGE electrophoresis and subsequently detected by fluorescent imaging immediately using an FX imager (Absorption at 495nm, emission at 519 nm, BioRad).
Fluorescence imaging uses external dyes or fluorescent markers which emit photons after excitation.
The lungs were excised, and red fluorescent protein-positive metastatic foci were imaged by fluorescent imaging (Leica MZ6).
Other techniques that may have value in clinical application include immunohistochemistry using EGFR mutation-specific antibodies [32] or the application of molecular beacons coupled by fluorescent imaging [33].
Cells were further visualized by fluorescent imaging.
Migration progress of the fluorescent jasmonate in tomato was first visualized by fluorescent imaging.
Arrays were developed by fluorescent imaging and captured with a digital imaging system.
After contacting and lateral scanning of the protease-immobilized AFM tip to the reporter peptide layer, a fluorescent area was observed by imaging using total internal refection fluorescence microscopy (TIRFM).
7 After intravenous injection of NIR fluorescent probes, we performed surgery guided by intraoperative imaging using a wide field-of-view imaging device capable of resolving microscopic clusters of tumor cells.
Recent work by Tian et. al. [ 16] used Monte Carlo simulations to study spatial resolution and depth dependent sensitivity to perturbations in both fluorescent and non-fluorescent two dimensional imaging using a homogeneous tissue geometry.
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