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RNA mass was determined by fluorescent detection using Qubit® Broad Range RNA kit (Life Technologies).
Total and free glutathione concentrations were assessed by HPLC method followed by fluorescent detection using a commercially available kit (Chromsystems Instruments & Chemicals, Munich, Germany).
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This limitation is fully addressed using real-time qPCR methods [ 54], whereby PCR product accumulation is monitored at each PCR cycle by means of fluorescent detection using molecular beacon chemistry.
Besides these, two new microsatellites in the MAML3 gene were genotyped by PCR amplification and capillary electrophoresis and fluorescent detection using an ABI Prism 3730 DNA Analyzer (Applied Biosystems).
Fluorescent cell clusters/features can then be analysed by simple fluorescence detection using common DNA array scanners or high-throughput microscopy, without the need for further manipulation of the slides such as immunofluorescence staining or enzyme-based detection.
YFP fluorescent cells migrating from the SVZ were detected in the cortex by fluorescent detection 24 hours after OGD.
We describe here a virus-based assay in which fluoromycobacteriophages are used to deliver a GFP or ZsYellow fluorescent marker gene to M. tuberculosis, which can then be monitored by fluorescent detection approaches including fluorescent microscopy and flow cytometry.
LAMP products were then evaluated by fluorescent detection with a fluorescent detection reagent (Eiken Chemical Co., Ltd).
Double fluorescent in situ hybridization was performed using DIG-UTP and FITC-UTP-labeled RNA probes followed by sequential detection using HRP-labeled anti-DIG and HRP-labeled anti-FITC antibodies (Roche, Indianapolis, IN, USA) and fluorescent tyramide amplification.
The algorithm for the fluorescent light detection uses these measurements and makes a decision about the existence of the fluorescent light in the scene.
Color compensation was carried out for multicolor detection using matched single fluorescent proteins compensation controls.
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