Sentence examples for by fluorescence polarization using from inspiring English sources
Stoichiometry of QacR(E90Q -R6G binding was determined by fluorescence polarization using a Beacon 2000 (Invitrogen) fluorescence polarimeter and excitation and emission wavelengths of 490 nm and 560 nm, respectively.
Binding curves were measured by fluorescence polarization using Pherastar (BMG Labtech, Ortenberg, Germany).
21 For Bim BH3 and Bmf BH3 displacement assays, 10 nM fluorescence-labeled Bim BH3 or Bmf BH3 peptide was incubated with 100 μM GST-Bcl-2 in binding buffer for 10 min. Bmf or Bim BH3 peptides were titrated and displacement of Bim BH3 or Bmf BH3 was detected by loss of fluorescence polarization using Safire2 plate reader (Tecan).
Displacement of the fluorescent ligand, which has high polarization when bound to the PPARγ-LBD and low polarization when not bound, was assessed by measuring loss of fluorescence polarization using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA).
Intracellular fluorescence polarization (IFP) values of normal human lymphocytes and leukaemic cells from newly diagnosed patients were determined from fluorescence polarization using fluorescein diacetate (FDA).
The former method exploits fluorescence polarization using a fluorophore-containing UDP-GlcNAc analogue.
Lidocaine concentrations were measured by fluorescence polarization immunoassay using a TDxFLx analyzer (Abbot Laboratories).
We determined the capacity of a new BH3 peptide mimetic, S44563, to displace a fluorescent Puma BH3 peptide from recombinant Bcl-2 or Bcl-XL by fluorescence polarization (FP) assays, using recombinant Bcl-2 or Bcl- XL and a fluorescent Puma BH3 peptide.
Even in the cases where our data overlap, the reported apparent KD values reported by Kaushansky et al. appear inconsistent with direct measurements conducted using well controlled solution binding measured by fluorescence polarization [ 57].
Validation of 60 binary interaction pairs was conducted using the orthogonal method of solution binding measured by fluorescence polarization.
Briefly, interactions were assessed using a peptide microarray followed by confirmation of positives and measurement of binding affinities by fluorescence polarization (FP), a high-quality affinity measurement method (Stiffler et al., 2007).
