Lipid uptake has been studied by fluorescence microscopy using suitably labeled fluorescent lipid analogs [20].
Cell labeling was monitored by fluorescence microscopy using a Nikon Eclipse E800 fluorescent microscope.
Phagocytosis was evaluated by fluorescence microscopy using thymocytes labeled with the green fluorescent dye CFSE and macrophages stained with PE-labeled IgG antibodies directed to CD11b.
We further tested the subcellular localization of Ga5DH in exponentially growing cells by fluorescence microscopy using polyclonal antibodies directed against Ga5DH.
The slides were examined by fluorescence microscopy using a Leica fluorescence microscope (Wetzlar, Germany) equipped with excitation/emission filter cubes for DAPI and FITC (fluorescein isothiocyanate).
Subcellular localization of exendin-4 in mouse pancreatic tumor cells was evaluated by fluorescence microscopy using fluorescein-Trp25-exendin-4 (ANAWA, Zurich, Switzerland) [29].
Mitochondrial morphology was inspected by fluorescence microscopy using a dsRED filter on a Zeiss Axioskop microscope.
The injected embryos were then examined by fluorescence microscopy, using a DM IRBE microscope (Leica, Wetzlar, Germany).
Determination of cell viability was performed by fluorescence microscopy, using the indicator Hoechst 33342 as previously described [40].
and sections analyzed by fluorescence microscopy using a Zeiss Axioplan microscope or Zeiss Confocal microscope (LSM 510 Meta DuoScan).
After washing with PBS cells were mounted onto glass slides and examined by fluorescence microscopy using a Zeiss (Jena, Germany) Axioscope2 plus microscope.
