Sentence examples for by fluorescence microscope using from inspiring English sources

(C) The cells were fixed and stained with anti-cytochrome c antibodies and analyzed by fluorescence microscope using appropriate filters for the visualization of green fluorescence resulting from the presence of FITC molecules.

Imaging was taken by fluorescence microscope using a Nikon TE2000s inverted microscope with 10x objective.

The number of apoptotic thymocytes inside each macrophage was determined by fluorescence microscope using PE-labeled IgG anti-CD11b.

Therefore, we analyzed the production of intracellular ROS in acrylamide-treated Caco-2 cells by fluorescence microscope using DCFH-DA.

Images were obtained by Olympus BX51 fluorescence microscope using Olympus DP72 camera and cellSens standard software.

Then the medium was replaced with PBS and after 20 min the fluorescence was directly visualised by means of a fluorescence microscope using a FITC filter (excitation wavelength of 485 nm and emission wavelength of 530 nm).

Each day, 30 μl of 2 mg/ml FDA and 0.1 mg/ml calcoflour white (Sigma-Aldrich, Canada) solutions were added to a single well and incubated in the dark for approximately 10 min. The stained cells were observed for cell viability as described previously, and for cell wall deposition by viewing on an inverted epi-fluorescence microscope using a DAPI/Hoescht/AMCA filter set (Chroma, Bellows Falls, VT).

Cellular adhesion on different surfaces was investigated by fluorescence microscope imaging using rhodamine phalloidin cytoskeleton stain and DAPI nucleus stain to identify adherent platelets and leukocytes.

After 4 hours, medium buffered with MES at pH 5.6, 5.0, or 4.6, was added for 10 minutes at 37°C and cell-cell fusion was monitored by fluorescence microscopy using an Axiovert25 fluorescent microscope 4 6 hours later.

To confirm that an optical detection of GFP fluorescence of TRI5prom:: GFP reporter strain resulted in a production of trichothecenes, the concentration of DON in ppb (parts per billion) of fresh weight of infected glumes and caryopses was quantified after GFP fluorescence of infected samples was observed by fluorescence microscopy using MZFLIII microscope.

The slides were examined by fluorescence microscopy using a Leica fluorescence microscope (Wetzlar, Germany) equipped with excitation/emission filter cubes for DAPI and FITC (fluorescein isothiocyanate).