Sentence examples for by fluorescence emission using from inspiring English sources
The cells were washed three times with PBS and fixed with 4% paraformaldehyde, and the cells stained with LY were detected by fluorescence emission using a reversed fluorescent microscope equipped with either a camera or a laser scanning confocal microscope (LSCM).
The reaction was monitored by fluorescence emission using a UV lamp.
This assay quantifies telomerase activity by measuring real-time fluorescence emission using real-time quantitative RT-PCR (qRT-PCR).
Labeling of aRNA was monitored by measuring the Cy3 and Cy5 fluorescence emissions using a nanodrop ND-1000 UV-Vis Spectrophotometer (BioRad).
The degree of LDL oxidation was monitored continuously by fluorescence emission at 234 nm using a fluorescence spectrophotometer (SLM-AMINCO® Series 2, Spectronic Instruments, Rochester, NY) and stopped when the fluorescence values reached the peak (≥1.1 fluorescence units).
The time course of unfolding of nonacetylated and acetylated γD-crystallin in 5.5 M GdnHCl, 10 mM phosphate buffer, 5 mM DTT, and 1 mM EDTA (pH 7.0) was monitored by fluorescence emission at 355 nm using an excitation wavelength of 295 nm in a FluoroMax-4P spectrofluorometer.
However, due to the poor biochemical information carried out by fluorescence emission spectrum, the use of Raman spectroscopy for biological tissue classification has been evidenced [ 17– 19]. Figure 4 shows fluorescence intensity (counts) against wavelength (nm) for a sample of rabbit's blood serum taken from control, HCD, and HCD + Zn groups.
The level of calcein fluorescence was monitored by fluorescence emission at 530 nm after exciting at 485 nm, using a Fluostar microplate reader (BMG-Labtechnologies, France).
Secondly, the vimentin concentration was determined using the Bradford test and dye concentration by fluorescence emission spectra.
The fluorescence spectra were measured by dispersing the emission using an Amici prism and detecting the spectrum with a CCD detector (Andor iDus DV 420A-BV).
Fluorescence emission detection is performed by dispersing the emission using an optical grating and collecting the dispersed emission using a PMT array.
