Sentence examples for by flotation on a from inspiring English sources

For this study, LBs were isolated from rat lung homogenates by flotation on a discontinuous sucrose gradient.

Transiently transfected COS-1 cells were lysed with Triton X-100 at 4°C and DRM's were separated by flotation on a sucrose gradient.

To confirm our microscopic observations, we performed biochemical analysis and proteomics on LDs isolated by flotation on a discontinuous sucrose gradient.

Separated, mixed life stage, nematodes were cleaned by flotation on a 30% (wt/vol) sucrose solution followed by three washes with 0.5× PBST [ 70].

Cells were resuspended in DMEM (Invitrogen) and purified by flotation on a density cushion of Percoll (30%) by centrifugation at 1800 ×  g for 20 min at 4°C.

After 24 hours of incubation, exosomes were purified from the 400 800 mL culture supernatant under endotoxin-free conditions by a series of centrifugation and filtration steps, followed by flotation on a sucrose cushion, as described in [ 18].

To further analyze the capacity of synphilin-1 to bind lipid rafts, we performed fractionations and separated the insoluble membranes by flotation on an Optiprep gradient.

Then 4.7 × 107, 4.6 × 107 and 4.3 × 107 peripheral blood mononuclear cells (PBMC) were isolated immediately by flotation on Ficoll-Hypaque.

The presence of PrPC in lipid rafts of 1C11 precursor, 1C115-HT or 1C11NE fully differentiated cells, was assessed using TritonX-100 insoluble glycosphingolipid (GSL) rich microdomains isolated by flotation on sucrose gradient and solubilized in 6% SDS.

Intact embryos were separated from debris by flotation on 30% sucrose.

These were removed by pipetting, washed in ddH20 and purified from debris by flotation on 60% sucrose.