Sentence examples for by five cycles of from inspiring English sources
The amplifications were carried out in a PTC-225 Tetrad machine (MJ Research, USA) with the cycling parameters of 2 min at 96°C followed by five cycles of 25 s at 96°C, 1 min at 70°C, 1 min at 72°C, then 21 cycles of 25 s at 96°C, 50 s at 65°C, 50 s at 72°C, and final four cycles of 25 s at 96°C, 1 min at 55°C, 2 min at 72°C.
The PCR program started with an initial step for five min at 95 °C followed by five cycles of 20 s at 95 °C, 20 s at 52 °C, and 60 s at 72 °C.
The lipid film was rehydrated with phosphate-buffered saline (PBS), and the resulting solution was sonicated in a bath sonicator followed by five cycles of freezing and thawing.
Moreover, both the Pt/MCNT and magnetic Pt-Fe3O4/MCNT catalysts show a good recyclability, confirmed by five cycles of reusage.
Cell lysate was prepared by five cycles of freeze thawing, and was used to sequentially infect the CP cells twice.
PBMC were lysed by five cycles of freezing and thawing, and stored at -80°C until assayed (within two months).
Two iterative cycles of magnetic bead selections followed by four cycles of fluorescence-activated cell sorting (FACS) were applied.
Nearly 17,500 private homes and 700 buildings containing 20,000 apartments were destroyed by two cycles of war.
About 50 concerts are planned in Mechelen, where Flemish music is complemented by three cycles of 20th-century music by the Paul Collaer Ensemble.
The as-synthesized nanoparticles were isolated by sonication and centrifugation (2,000 g) in dry toluene, followed by three cycles of washing and centrifuging in 2-propanol (IPA).
