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The reaction rate constant evaluated by fitting the rate data to the model prediction was 6.50 m3 / (kmol s) at 303 K.
The kinetic parameter Km and Vmax were determined by fitting the rate of reaction versus substrate concentration curves to the Michaelis-Menten equation, ν/Vmax = [S]/([S]+Km) using a nonlinear regression analysis in Graphpad Prism software version 4.0 (GraphPad software, La Jolla, CA, USA).
Steady-state kinetic parameters were determined by fitting the rate data to the Michaelis equation.
Vmax and Km were determined by fitting the rate of formaldehyde formation at each reductase concentration as described previously.
To obtain dose-response curves, we calculated resazurin reduction rates for each concentration of A2E or retinaldehyde by fitting the rate of resorufin appearance, from 40 min to 150 min, to regression lines (using GraphPad Prism, GraphPad Software, La Jolla, CA, USA).
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Kinetic parameters (kcat and KM) were determined by fitting the initial rate data to the equation V0 = (Vmax[S])/ KM + [S]), where V0 is the initial rate and [S] is the substrate concentration, using Origin version 6.0 (OriginLab Corp., Northampton, MA).
The RSM was used in practice for determining the kinetic models by fitting the initial rate dates based on the equations of ping-pong bi bi and order bi bi model.
The mechanism of Qu release from PMs was performed by fitting the release rate data into the following equations: Zero ‒ order model equation : y = k 1 t + a 1 (1) First ‒ order model equation : ln 100 - y = k 2 t + a 2 (2) Higuchi ' s square ‒ root equation : y = k 3 t 0.5 + a 3 (3).
The kinetic parameters were obtained using GraFit version 5.0.11 by fitting the initial rate data to eq 2: 2where K0.5 is the dissociation constant and n is the Hill coefficient.
Steady-state kinetic parameters were determined by fitting the initial rate data to the Michaelis Menten equation, using the enzyme kinetics module of SigmaPlot version 9.0.1 (Systat Software , Inc.
The steady-state kinetic parameters were obtained using GraFit version 5.0.11 by fitting the initial rate data (n = 2) to eq 1: 1tobtainin the values reported in Supporting Information Table 3.
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