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Rates of calcein release (which corresponds to pore formation) were calculated by fitting the normalized fluorescence intensity data with a nonlinear fitting algorithm in OriginPro 8.6.
On the other hand, by fitting the normalized experimental data by means of the classical low-pass filter formula.
The voltage-dependent activation of the currents was analyzed by fitting the normalized current/voltage relation by the Boltzmann function.
We estimated the equilibrium concentration of free actin monomers Feq, the dissociation rate koff and the association rate k*on by fitting the normalized experimental FRAP curves with equation (3) (see Materials and Methods).
Based on this model, we estimated the equilibrium normalized concentration of free WT-L-plastin molecules Feq (Feq WT) = 0.513±0.01) and the association rates k*1on (k*1on(WT) = 0.338±0.032 s−1) and k*2on (k*2on(WT) = 0.01±0.001 s−1) by fitting the normalized experimental FRAP curves with equation (5) (Fig. 1E, see Materials and Methods).
IC50 values were calculated by fitting the normalized inhibition data to eq 1: 1usinGrafitit 5.0.1.1 where [I] is the concentration of inhibitor.
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It is shown in this paper that the estimation of these quantile functions can be achieved by fitting the tail of the normalized performance index.
However, we were able to compare the goodness of fit by comparing the normalized χ values obtained by fitting the models to our data (χ normalized by the number of fitting parameters; Roberts et al. 2007).
We extract the visibility of the normalized curve by fitting the data, as described in the Methods section, and we find that the visibility is Vo=0.038±0.001.
The EXAFS spectra [χ k)] were extracted from the normalized absorption spectra by fitting the region between k = 1 and ca. 10 or 12 Å–1 with a spline function.
Second, normalization was performed by fitting the array-specific local regression model of M as a function of A. Normalized log-intensities for the two channels were then calculated by adding half the normalized ratio to A for the Cy5 channel and subtracting half the normalized ratio from A for the Cy3 channel.
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by fitting the related
by integrating the normalized
by summing the normalized
by indexing the normalized
by fitting the experimental
by using the normalized
by fitting the observed
by fitting the new
by evaluating the normalized
by fitting the corresponding
by finding the normalized
by adding the normalized
by fitting the genomic
by monitoring the normalized
by subtracting the normalized
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