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· P x bleach ), this order can be gained by fitting the half-life period exponentially against multiple mean powers.
By fitting the Monte-Carlo simulation to the experimental half-times of crystallization the surface nucleation concentration and the bulk nucleation rate was estimated at 4 different temperatures.
The concentration of drug needed to reduce plaque numbers by half (IC50) was calculated by fitting the data to a linear regression model using Prism software (GraphPad Prism Software, Inc., La Jolla, CA).
The effective half-life was evaluated by fitting the radioactivity concentration values of the late PET images (i.e. 4 and 24 h after injection) with a mono-exponential curve.
The enzyme half-life was calculated by fitting the activity data to exponential or linear functions.
Recovery half-times were calculated by fitting the curves to a mono-exponential equation with plateau set at 100%.
C50 values (relative tonicity at half maximal lysis) were calculated by fitting the data to a 4-parameter logistic sigmoidal curve.
The first order rate constants (kinact) for enzyme inactivation were obtained by fitting the results to an exponential function (Fig. 3) and half lives calculated from t½ = 0.69/kinact.
The half-life of the displacement curves was determined by fitting the curve with Prism software (Graphpad).
By fitting the dose response curve to the Hill equation, apparent calcium sensitivity was derived as the half maximal effective concentration (EC50) of calcium required for channel activation.
By fitting the PL spectra with a Gauss peak, the emission peak energies, spectral linewidths [full width at half maximum (FWHM ], and emission intensities are determined.
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