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The first-order reaction rate constant for the reaction was determined by fitting the fluorescence intensities of the samples to the first-order equation: {text{Ln}}(I_{t} ) = - k_{1} t + {text{Ln}}(I_{0} ),where I t and I0 are the fluorescence intensities at time t and at the initial time.
In each image, we determined the position of the QDs with a ∼30 nm resolution by fitting the fluorescence spots with a 2D gaussian curve [58].
Figure 5 shows that the model, whose parameters are constant and obtained by fitting the fluorescence distribution curves, quantitatively predicts the observations in E conditions, and qualitatively matches the experiments in S conditions.
The second row contains parameters obtained by fitting the fluorescence data under the constraint that m = n.
The characteristic recovery times and fluorescence recovery plateau (Fp) were calculated by fitting the fluorescence recovery curves as previously described (Soumpasis, 1983; Braeckmans et al., 2003).
The absolute calibration to areal densities (μg/cm) was achieved by fitting the fluorescence signal from thin-film standards SRM-1832 and SRM-1833 (National Institute of Standards and Technology, Gaithersburg, MD).
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Rates of calcein release (which corresponds to pore formation) were calculated by fitting the normalized fluorescence intensity data with a nonlinear fitting algorithm in OriginPro 8.6.
The observed rate constant, kobs, was obtained by fitting the emitted fluorescence at 530 nm to a single exponential decay.
The first row contains parameters obtained by fitting the GFP fluorescence data (F) with the four-parameter model described in the text.
Therefore by fitting the logarithm of fluorescence intensity versus the logarithm of the laser power, the slope value indicates the number of photons absorbed to excite the fluorescence.
Rate constants for spontaneous emission, cross-relaxation, and up-conversion were determined by fitting the model to fluorescence lifetime data at 300 K, a temperature at which multi-phonon relaxation can be neglected.
More suggestions(15)
by integrating the fluorescence
by fitting the strain/time
by dividing the fluorescence
by fitting the sample
by measuring the fluorescence
by normalizing the fluorescence
by fitting the logδ φ
by fitting the model
by using the fluorescence
by monitoring the fluorescence
by fitting the payment
by recovering the fluorescence
by evaluating the fluorescence
by quantifying the fluorescence
by comparing the fluorescence
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