Sentence examples for by fitting the absorbance from inspiring English sources
The concentrations required to give 50% inhibition (IC50) were estimated by fitting the absorbance data to a logistic curve with SAS version 8.2 (SAS Institute, Cary, NC, USA).
The rate of the first electron transfer was determined by fitting the absorbance traces at 450 nm with multiple exponentials using KinetAsyst software (Hi-Tech).
Once each patch was aligned across all the acquisitions in a particular experiment, the multispectral images were processed by fitting the absorbance at each pixel to the model described by Eq. (3).
Values for the individual rate constants in the kinetic mechanism of CpIMPDH were derived by fitting the absorbance and fluorescence stopped-flow progress curves using the program DynaFit (22) as described in the Supporting Information.
The observed pseudo first-order rate constant (k obs) was determined by fitting the absorbance decay resulting from association of the protein with CO, to a single exponential function.
The generation time was calculated in R (R development core team 2011) by fitting the absorbance measurements that belonged to the exponential phase to a linear model, and then dividing log(2) by the obtained coefficient.
The rate of NADH consumption was calculated by fitting the initial absorbance change at 340 nm to a linear function.
Dose response curves were then generated by fitting the final absorbance ratios using Prism 6 software (Graphpad, CA, USA).
The observed rate constant for the first process can be obtained by fitting the change in absorbance at 402 nm over the first second to the following equation: kobs = A0,402 + Af,402(1 – exp(− kobs t).
By fitting the time evolution of the peak absorbance with a first-order kinetic model, the photoswitching rate constant was calculated to be 1.17 × 10−2 s−1, which is comparable to the reported value of 9.6 × 10−3 s−1 using 405 nm 1 mW/cm picosecond laser by Hofkens et al [ 10].
The limit of detection (LOD with S/N = 3) for JAI-51 was 3.85 × 10-7 M and the linearity of the calibration curves was prepared by fitting absorbance vs. amount of JAI-51 standards between 3.85 × 10-7 M and 20 × 10-7 measuredred 3 times).
