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In particular the Ct values were normalized by fitting an analysis of variance (ANOVA) model that accounted for "within primer across treatment" and "within treatment across primer" variation.
When the Z-scores were analysed to assess for differences between participants, data were adjusted/controlled for BMI, 36 by fitting an analysis of covariance to the data.
Between-group differences in GMV were estimated by fitting an analysis of covariance (ANCOVA) model at each intracerebral voxel in standard space.
The relationships between herkogamy, dichogamy and autofertility at the plant level were analysed by fitting an analysis of covariance model where population was included as a categorical variable, and herkogamy and dichogamy as covariates with unique slopes for each population.
For the fMRI data, the effects of group, time (pre-intervention and 2 weeks' post-intervention) and task (0-back and 1-back conditions) will be examined using factorial analyses by fitting an analysis of variance (ANOVA) model [ 81]. fMRI effective connectivity will be examined using Dynamic Causal Modelling [ 82].
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GLA1BLUP EBV were obtained by fitting a linkage analysis based relationship matrix, G LA1, [ 14, 15] for which the probabilities of paternal/maternal inheritance were obtained using the LDMIP program [ 6].
In the generalized linear model, framework discriminant analysis was carried out by fitting a binary regression analysis using R statistical package.
The ADC can also be calculated using a multi- b analysis by fitting an exponential function to the measured signal intensities on multi- b acquired images [ 34].
The power of the ASSET model to explain individual cost variability was determined by fitting a linear regression analysis using the log transformed actual individual costs reported by 50,000 subjects randomly drawn from the sample, as the dependent variable and the log transformed mean costs by age group as the independent variable.
Statistical analysis was conducted by fitting an unpaired two-tailed Student's t test, as described above.
We attempt to resolve this issue by introducing parameter uncertainty into our analysis – by fitting a gamma distribution to the deterministic estimates of Pc as well as PAc, and running 1000 Monte Carlo simulationsa for each product.
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