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The IC50 values were determined by fitting a sigmoidal dose response curve on GraphPad Prism.
The EC50 was determined by fitting a sigmoidal dose response curve on GraphPad Prism.
The maximal effective concentrations (EC50) were determined by fitting a sigmoidal dose response curve on GraphPad Prism (GraphPad Software).
The half-maximal inhibitory concentration (IC50) values were determined by fitting a sigmoidal dose response curve on GraphPad Prism.
The IC50 was calculated by fitting a sigmoidal dose response curve using Sigma Graph Pad Prism® version 6.0.
ELISA data were analyzed by subtracting the plate background from all readings and by fitting a sigmoidal dose response curve to the data for each sample by using the Prism software (Graphpad, San Diego, CA).
Similar(48)
For each data set, the total fraction of unreplicated DNA (1-ftotal(t)) was obtained by fitting a Boltzman-sigmoidal function to the temporal changes in the relative DNA content (data points shown in Figure 2A, right panel, in [4] and in Figure 1D in [5]) using a Levenberg-Marquardt algorithm.
Fitting a sigmoidal curve to cubic data gives nonsensical results (Myhrvold, 2013).
Fitting a sigmoidal curve through the data allows the circadian rhythm component of the model to be eliminated.
The onset of export across the PVM was subsequently determined, using the second derivative maximum method (Luu-The et al., 2005), and the export rate was calculated by fitting a linear function to the linear part of each sigmoidal curve.
Apparent ABA affinities (K′d) were calculated from emission ratio plots by fitting a four parameter logistic curve R = R m i n + R m a x − R m i n 1 + ([ A B A ] K ' d ) n or from ΔR/ Δ Rmax plots by fitting a three parameter sigmoidal Hill equation Δ R Δ R m a x = R m a x · [ A B A ] n K d ′ n · [ A B A ] n (Palmer et al., 2006) using the SigmaPlot 10.0 version (Systat, San Jose, CA).
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by introducing a sigmoidal
by fitting a cubic
by fitting a common
by fitting a univariate
by fitting a standard
by fitting a polynomial
by fitting a 2D-Gaussian
by fitting a mixed
by fitting a computational
by fitting a quartic
by fitting a gamma-variate
by comparing a sigmoidal
by fitting a linear
by fitting a parametric
by fitting a joinpoint
by fitting a relaxed
by fitting a horizontal
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