Sentence examples for by first treating the from inspiring English sources

The problem of quenching can be partially resolved by providing the magnetic nanoparticle with a stable shell prior to the introduction of the fluorescent molecule, or by first treating the fluorophore with an appropriate spacer.

The combined effect of Stargen™ 002 and Carezyme™ was studied by first treating the cell lysate with Stargen™ 002 followed by Carezyme™ treatment both in the conditions described above for each individual enzyme.

Alternatively, combining sonication and lysozyme treatment was carried out in M5 by first treating the pelleted protoplasts with lysis buffer (50 mM Tris HCl pH 8, 150 mM NaCl, 2 mM EDTA, 1% Triton, 1% PMSF) and then sonication for 30 min at 60% amplitude.

The chip was then bonded to the glass slide by first treating the PDMS surfaces with oxygen plasma from a BD-10ASV spark coil Electro-Technic Products Inc.., IL, USA) for about 30 s.

Protein digestion was performed by first treating the protein extract with urea (8 M) and DTT (4.5 mM) at 60°C (1 h) to denature the proteins and cleave the disulfide bonds, followed by 10× dilution with 50 mM NH4HCO3 and trypsinization at 37°C for 24 h (50 1 w/w protein:enzyme ratio).

Notably, changing of the sequence of drug administration by first treating the cells with NU1025 for 24 h, washing, then treating with C-1305 + NU1025 for 48 h (Fig. 4A, right panel) alleviated the anti-proliferative effect of the TOPO II inhibitor.

In the previous experiments, combination treatments were performed in a sequential format by first treating cells with a sensitizing drug for 24 hours, removing the drug from the cells, and then applying a TRAIL treatment for an additional 24 hours.

Vinyl fluoride is prepared from acetylene and hydrogen fluoride by direct reaction in the presence of HgCl2 or by first treating acetylene with excess hydrogen fluoride to form CH3CHF2, which is then converted to H2C=CHF by heating at 700 °C (1,300 °F).

A diversity library was developed by first treating 16 with phthalic anhydride to generate phthalimide 17 in 88% yield.

This was tested by first treating with either diclofop-methyl or chlorsulfuron, observing plant survival (resistant plants) then treating with diclofop-methyl or chlorsulfuron plus malathion.

Randomization was stratified by CD4 cell count (< or ≥50 cells/mm3) and by first treated OI/BI at study entry (PCP, vs. BIs, vs. all other OIs).