This model M generates a network G by first sampling the membership of each vertex u with probability π k for cluster k, then sampling each edge e uv =0 or 1 as a Bernoulli trial with success probability θ ij for u∈ i and v∈ j.
MR: And this is just the first sampling with more coming?
Third, samples with one or less milligram carbon (mgC) can be analyzed by AMS.
Data were collected by a stratified two-stage sampling technique, with 888 primary sampling units (PSUs) selected in the first sampling stage, and 7,864 households selected through probability sampling in the second sampling stage.
In addition, this hypothesis is further supported by the fact that the samples inoculated with either prep1 or prep2 were shown to encompass a higher number of strains at the second sampling time compared to the first sampling (4dd = first sampling in Table 3).
NA is extracted from the remainder of the PBS diluted saliva sample by first mixing with Pluronic lysis buffer and then running the lysed sample over the NA-binding silica membrane.
Briefly, auto-inactivation of GAD was performed by first incubating GAD samples with 20 μM PLP for 5 min at 37°C, excess PLP was removed by applying the GAD sample to a 5 ml HiTrap™ desalting column (GE Healthcare) in 100 mM K/NaHPO4 (pH 7.2), 1.0 mM 2-mercaptoethanol, 1.0 mM AET and 5% (v/v) glycerol.
Each FORC was measured by first saturating the sample with a positive applied field of 1 T and then ramping down the field to a reversal field, then measuring the magnetization as the field increases from the reversal field back to positive saturation.
The wet weight of the sample scaffold was calculated by first blotching the sample surface with filler paper to remove adsorbed water, then weighing quickly.
The bootstrap samples are formed by first choosing with replacement from the two replicate 96-wells plates and by then choosing with replacement wells of each type (i.e., media-only, fibroblast, mono-culture, co-culture) from the chosen plates.
