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Quality control of the pooled library was monitored by first observing a single band on an Agilent bioanalyzer using a High Sensitivity DNA Kit (5067 4627; Agilent) for double-stranded DNA and last by determining molecules per microliter using quantitative PCR techniques (KK4821; KAPA Biosystems).
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The relative abundance of components in unknown mixtures were calculated by first observing the mean target RNA fraction for the neat components across replicates.
Determine the surface runoff by first observing if the soil surrounding the pit has a ground cover or if it is left bare.
This is done by first observing that in this wider Mach number range the shock cell structure in the jet plume usually begins with a strong shock followed by a quasi-periodic shock cell structure.
Data collection therefore was mainly driven by first observing the events, the subsequent interviews with the participants to gain insight into the context of activities and reasons for the associated information exchange.
The fact that photoluminescence can also be excited by ultraviolet radiation was first observed by a German physicist, Johann Wilhelm Ritter (1801), who investigated the behaviour of phosphors in light of various colours.
They start with an economic law first observed by a 19th-century statistician, Ernst Engel: richer households spend a smaller share of their income on food.
A tropical storm was first observed by a ship on September 8 while located about 750 mile east of Barbados.
The ins and outs of post-seismic ionospheric disturbances were first observed by a high-resolution two-dimensional GPS-TEC observation.
To simplify matters, we first observe by a symmetrizing argument (see Sect. 5) that there is a network storing all k-cliques if and only if there is one with constant threshold (theta = (z, ldots, z) inmathbb{R}^{n}) and satisfying for each pair (e neq f), ether (W_{ef} = x) (whenever e and f share one vertex) or (W_{ef} = y) (when e and f are disjoint).
In 1859 Kirchhoff and the German chemist Robert Wilhelm Bunsen discovered two new elements cesium and rubidium by first observing their spectral lines.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com