Sentence examples for by first isolating the from inspiring English sources

This was achieved by first isolating the DNA-bead mixture on a SPRIPlate and then discarding the derived buffer as per the manufacturer's protocol.

In situ contraction experiments were performed by first isolating the tibialis anterior (TA) muscle under anesthesia (ketamine/xylazine) from both legs and connecting the distal tendon of one leg to a force transducer with string.

This was achieved by first isolating the A- and D-genome homoeologous reads from both allotetraploids, G. barbadense and G. hirsutum, ("AT" and "DT" hereafter) using EST sequences from the A- and D-genome diploid species as a guide (denoted by "A2" and "D5" hereafter).

To screen for the desired editing event, genotyping of multiple exconjugants was carried out by first isolating the genomic DNA and then PCR amplifying the target locus.

Expression analysis was achieved by first isolating total RNA, followed by the generation of cDNA, which was subsequently used in RT-qPCR.

Next, to quantify the number of sperm stored by each male, we first isolated the seminal glomera from males collected for testis size examination, measured the fresh weight (wet mass) of the left and right seminal glomerus to the nearest 0.01 g using an electronic balance (Ohaus Navigator), and calculated the combined seminal glomera mass as the sum of the left and right glomerus mass.

The English chemist Henry Enfield Roscoe first isolated the metal by hydrogen reduction of vanadium dichloride in 1867, and the American chemists John Wesley Marden and Malcolm N. Rich obtained vanadium of 99.7 percent purity by a calcium reduction process in 1925.

We were able to detect both forms in similar amounts by first isolating glycoproteins from the medium of untransfected cells using concanavalin A, followed by immunoblotting using an antibody that recognizes both forms of the protein.

This difficulty may be overcome by first isolating immune complexes (IC) from the serum and using this fraction for testing.

CD4+CD25− naïve T cells were purified by first isolating CD4+ T cells from the venous blood of healthy donors using RosetteSep human CD4 T cell enrichment mixture (StemCell Technologies, Vancouver, Canada).

Isolated α-cellulose was generated by first isolating holocellulose from milled biomass pulp using the method described above.