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Monocular enucleation (ME) was performed by first inserting a micropipette in the developing eye in order to favor the holding and then the removal of the eyeball by tweezers.
Voters using this system vote by first inserting a punch card with perforated rectangles into a plastic marking unit that contains ballot pages.
The fixator was applied orthogonally to the femur by first inserting a 6 mm diameter Schanz screw (5 mm in small diameter femurs) reference pin at the subtrochanteric level near the lesser trochanter, perpendicular to the bone and in the coronal plane.
Dual expression vectors containing the CNOT6L and CNOT7 cDNAs were generated by first inserting a PCR-generated CNOT7 cDNA fragment containing a 5′ BamHI and 3′ SalI restriction site into the BglII and XhoI sites of multiple cloning site 2 of vector pACYCDuet-1 (Merck Millipore).
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The effect of methylation of the ITGAL promoter 5' flanking region was determined by first inserting an NdeI site into the ITGAL promoter at bp -382.
An edit script transforming s0, i -1 to t0, j can be composed by first inserting the letter s i -1 at the end of s0, i -1 to obtain the string s0, i at cost ins (s i -1), and then transforming s0, i to t0, j by applying an optimal script at cost e d s0, i, t0, j ).
Waterproofing of the device will be achieved by first inserting it into a nitrile finger cote and then wrapping the device in waterproof Opsite Flexifix (Smith & Nephew).Thereafter, the activPAL3 will be secured to the anterior mid-line of the right thigh, about a third of the way down from the hip, using hypoallergenic adhesive material (Hypafix®, BSN medical).
At the moment, companies are making antibody drugs by first inserting the gene for a specific antibody into cells culled from hamster ovaries and then growing the cells by the trillions in enormous vats, through a fermentation process not unlike that used to make beer.
The GFP/PiCdc14 construct was constructed using pTOR as a backbone, by first inserting the Cdc14 promoter into its KpnI-ClaI sites, then inserting GFP taken from pSAM into EcoRV-NotI sites, and finally placing the Cdc14 ORF into the NotI site.
MD simulations on dimer S-MB were conducted here by first inserting the lowest energy conformer from the above RosettaDock computations into a SDS-water mileu with a detergent/peptide molar ratio of 28/1.
E-stim is administered by first inserting needles via regular acupuncture (points) and then attaching wires to the needles with clips.
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CEO of Professional Science Editing for Scientists @ prosciediting.com