In the methodology of PLATON connected sets of atoms are assembled by first fixing a suitable atom of the molecule of the greatest molecular weight.
Frozen tissues were prepared for IHC by first fixing them in 10% neutral buffered formalin (Sigma) for 16 h at 4°C, subject to ThermoShandon tissue processor and embedded in paraffin.
staining for Lck and Fyn in addition to IL-17A, TNFα and IFNγ was performed by first fixing cells in a final concentration of 1.5% formaldehyde for 10 min at 37°C.
Two different types of ELISA were performed – first, by fixing the phage to the plates and testing against the plasma of patients and controls.
Second, by fixing the flaws in WTO provisions so that the trading rules are implemented fully and equitably.
Second, by fixing the mean structure, the forward stepwise method is again applied to determine the time lags for the sigma parameter in Eq. (6).
In the second and third steps, by fixing the common non-reactive flow and transport parameters, the sorption parameters (retardation factor, sorption coefficient, and kinetic rate constant) of each model are estimated using the breakthrough data of reactive tracers, neptunium and uranium, respectively.
The effect of rotation angle of the spiral antenna was first studied by fixing the line width to 1 μm and setting the rotation angle to 360*n (the rotation angle starting from the center of the spiral antenna, n changed in 1~2.8) in the simulation.
Indeed, if at some moment in time, we have a word with fitness 5 say, then we choose our next word by fixing the first and third letter (since these are already correct), and choosing a new second letter at random among all remaining possibilities that we have not been seen before.
