Sentence examples for by first digesting the from inspiring English sources

Fresh fd-RP vector is the shorter vector prepared by first digesting the fd-RP DNA with BamHI and ligating the resultant vector fragment, yielding a construct with a truncated SfiI fragment [18].

Primary cultures of brown adipose and subcutaneous, mesenteric, and gonadal white adipose tissues were generated by first digesting the tissue and pelleting the SVF, as described above.

The assay utilizes a strategy which reduces the complexity of human genomic DNA up to 10 fold by first digesting the genomic DNA and then ligating STY adaptor sequences onto the DNA fragments.

Moreover, the insoluble nature of the matrix complicates analysis of intact proteins, a challenge that can be overcome by first digesting the matrix before MS of the resulting peptide mixture.

Generation of the pIN-G construct (Fig. 1B) was achieved by first digesting the pEGFP-C1 vector (Clontech, Palo Alto, CA) with Age I (bp 601) and Sal I (bp 1370) to yield a 769 bp fragment, corresponding to the coding region of EGFP.

This was accomplished by first digesting the 130mer ssDNA library, which contained an internal uracil at the barcode junction from the previous PCR (see Figure 1), with an uracil DNA glycosylase, endonuclease VIII, which removes the uracil from the ssDNA strand.

We attempted to enhance the sensitivity of SNaPshot to detect the mutant by first digesting ER-α exon 4 with the MboII restriction enzyme, which cleaves only the wild-type sequence.

Total P in both original sample materials and their extracts (TPE) was obtained by first digesting samples in concentrated H2SO4 and HClO4 for 2 h and the measuring total dissolved P in digested solutions by a UV-vis spectrometer (Lambda 25, Perkin Elmer, Waltham, MA, USA) by the molybdate colorimetry method [17].

We eliminated the GFP gene from LV-HA-TrkB/GFP by first digesting with EcoRI and, because two NheI sites are present in this plasmid, partial digestion with NheI afterwards.

As described previously [22,26] purified type 1 astrocytes were prepared by first digesting cortices (dissected from 1 day old Sprague Dawley rats) in 1.33% collagenase (Sigma, Poole, UK), seeding (∼2 × 10 cells per T75 flask) and culturing the cells in poly- l-lysine coated T75 flask for 10 12 days.

Dried plant samples were first digested by the method given by Allen et al. [ 13]. 0.5 g of dried plant samples was taken in digested by nitric acid : perchloric acid (2 : 1).