After fingerprinting and sequence analysis, we obtained 27 unique VHHs (Fig. 1).
However, genetic variations are known to occur within M. mycoides subsp. mycoides SC as evidenced by restriction fragment length polymorphism [3,4], by IS 1296 and IS 1634 fingerprinting [5 7], and by multilocus sequence analysis [8].
Similar groups of clones identified by restriction digestion fingerprinting were also identified by HRMA and were confirmed by nucleotide sequence analysis.
All groups identified with restriction digestion fingerprinting could also be identified by HRMA and were overall confirmed by sequence analysis.
Mutants were confirmed by DNA sequence analysis.
The Kidd et al. (2008) study reported a total of 224 inversion, 724 insertion and 747 deletion variants, which were validated by fingerprint analysis, clone sequencing or FISH.
DNA sequences of the constructed plasmids were verified by sequence analysis (Eurofins Genomics, Tokyo, Japan).
These were verified by sequence analysis.
The isolates were characterized as atypical strains of C. meningosepticum by complete biochemical investigation, 16S rRNA gene sequence analysis, cellular fatty acid analysis, and random amplified polymorphic DNA fingerprinting (RAPD).
Sequence analysis of the cloned bands obtained by PCR fingerprinting indicated that if the same or extremely similar, inversely oriented tandem repeats are located close to each other, when only one repeat-specific primer is used in the PCR, the region between these repeats is amplified.
