Sentence examples for by filtration on a from inspiring English sources

Ten millilitres of the sample were first put into a sterile screw-capped Falcon tube, and then, a one-litre sample of water was concentrated by filtration on a 0.20 μm pore-diameter cellulose membrane filter.

Pillai et al. ([2011]) segregated clumps of cells using a ground glass grinder followed by filtration on a nylon mesh prior to microscopic observations of Pseudostuga menziesii (Mirb).

PCR products were purified by filtration on a MultiScreen®-HV plate (Millipore) loaded with Bio-Gel® P-100 Gel solution Bio-Rad Laboratoriess). Bio-Rad Laboratories

The membrane solids were recovered by filtration on a Buchner funnel lined with a Whatman No. 1 filter paper, and then washed with 5×5 mL aliquots of 0.1% v/v acetic acid to remove excess DTT.

Single pancreatic cell suspensions were obtained by 30 min digestion in 1.6 mg/ml collagenase type IV (Whorttington), followed by filtration on a 70 μm nylon mesh.

Methanolic extracts were then collected by filtration on a Whatman no. 1 filter paper disc and concentrated by rotoevaporation to a final volume of 80 mL (aqueous fraction).

The membranes were collected by filtration on Glass Fibre filters, Filtermat A (Perkin Elmer, USA), using a TOMTEC Mach III cell harvester (Orange, CT, USA).

The protein, with 99%% homology to human HMGB1, was purified with Ni Sepharose High Performance affinity media (GE Healthcare, Chalfont St. Giles, UK) according to the protocol supplied by the manufacturer, followed by gel filtration on a Superdex 75 column (GE Healthcare) with phosphate-buffered saline (PBS), pH 7.4, as running buffer.

For binding assays, Cry1Ca was further purified by anion-exchange chromatography followed by gel filtration on a Superdex-75 column containing 1 mM dithiothreitol (DTT) according to published procedures [ 50, 51], to remove the adsorbed toxin fragments that could interfere in the radiolabeling of the toxin.

The homogeneity of the second fraction (SE2) was estimated by gel filtration on a Superdex 75 10/300 column by FPLC using an AKTA Purifier (GE Healthcare).

The labeling mixture was purified by gel filtration on a 5-ml G25M column, by reversed-phase high-performance liquid chromatography (RP-HPLC) using a C8 column or by solid phase extraction (SPE) on an HLB cartridge.