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Atherosclerotic plaques were visualized by fat staining using Oil red O (ORO).
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Our findings, together with other independent reports, strongly suggest that vital staining using fat-soluble dyes including both Nile Red and BODIPY, should not be used to assay fat stores in living C. elegans.
We find that fixative staining using Sudan Black, Oil Red O, and Nile Red produce consistent and reproducible data on fat stores (Table 1).
Protein concentration was determined by Coomassie stain using BSA standards.
Additionally, sections of the mammary fat pad tumors were stained using H&E.
Cells were stained using the fat-specific dye oil red O to monitor lipid accumulation at various days of differentiation (Fig. 1B, C).
We used fat staining of fixed nematodes and biochemical lipid analysis to demonstrate considerable differences in fat stores in C. elegans feeding on various E. coli strains.
Histological sections were stained using hematoxylin and eosin stain (H&E stain) and observed by light microscopy.
Furthermore, questions have been raised as to which lipid species are stained by commonly used fat stains in genome wide screens of fat metabolism.
First, the cream turned brown and separated from the fat, staining the cellophane wrapper.
Right: representative images of fat staining.
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