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In hippocampal CA1 pyramidal neurons either extrasynaptic α5 or δ containing GABAA channels have been shown to carry the small tonic current that may be present in the neurons at basal ambient GABA concentrations [8], [9], [18], [19], [22] and increases when the extracellular GABA concentration is elevated by external applications of GABA [9], [14], [18].
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Plant root morphology is known to be influenced by water and nutrient availability as well as by external application of hormones (Lopez-Bucio et al. 2003).
The transcription initiates when the gene is induced in defense response, a condition that can be simulated experimentally by external application of salicylic acid.
Removal of DTT by external application of 100 µM Cd2+ to oocytes expressing I227C channels, rapidly converted the slow voltage-dependent activation into instantaneous leak K+ current (Figure 4B, lower inset; τact slow = 981±138 ms vs. τact fast = 90±10 ms, at +40 mV, n = 10), with an increased Iinst/Imax ratio (Table 1).
Activation of TRPM8 currents was achieved by external application of 20 μM Icilin.
In some species and varieties, seedless fruit production is often achieved by external application of plant growth regulators as in the case of grape, tomato and eggplant [ 3].
Experiments show that the resting ouabain-insensitive sodium efflux in giant fibers from the barnacle Balanus nubilus is stimulated by external application of pentachlorophenol (PCP).
In pea (Pisum sativum) and Lotus japonicus, strigolactone deficient mutants were shown to produce less nodules, which could be rescued by external application of GR24 [ 2– 4].
The role of cuscutain and its inhibitor during the host parasite interaction was studied by external application of an inhibitor suspension, which induced a significant reduction of successful infection events.
To test if the phenotypes observed could be rescued by external application of BR, adult 35S p :c-Myc-CES-SRDX/203 plants were sprayed twice a week with a 1 μM 24-epiBL solution.
Voltage protocols were generated and electrical signals were digitized at 5 kHz using a DigiData 1200 or a Digidata 1322A hosted by a Pentium PC running either pCLAMP 6.0 or pClamp 8.2 software (Molecular Devices Co .. Muscarinic cationic current (mIcat) activated by external application of 10 μM CCh (see above) was recorded at holding potential of −50 mV.
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