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Second, the effect could be rescued by expression of an siRNA resistant form of cortactin in Rap1(Q63E -expressing cells.
Several tumor and cancer models were generated, by expression of an oncogene driven by a tissue-specific or ubiquitously expressed promoter (for a review, see Mione and Trede, 2010).
However, the ability of epigenetic drugs to stimulate metastasis in RMS cells was inhibited by expression of an Ezrin-specific shRNA.
Removing one (not shown) or both copies of Mkk4 does not suppress the small eye phenotype induced by expression of an activated version of hep (hepCA) in the Drosophila eye [12] (Figure 1N and O).
The importance of the missing tail region from the P100 R4227X mutant was confirmed by expression of an artificial PC1 product, AESW, containing only the final transmembrane domain and the C-terminal tail in Xenopus oocytes.
Microtubule orientation was fully rescued by expression of an RNAi-resistant oMAP4 construct.
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It is now clear that DM is not caused by expression of a mutant protein.
We show that cells lacking Gpx3 have aberrant mitochondrial morphology, defective protein import capacity, and lower inner membrane potential, all of which can be rescued by expression of a mitochondrial-only form of Gpx3.
All attempts to rescue the dvps4Δ7b mutants by expression of a dVps4 wild type construct failed.
One may be concerned that such oscillations might thus be precluded by over-expression of a protein (whose transcriptional level could then not be regulated anymore), or by expression of a constitutively active form of the protein.
Our findings are in contrast to those obtained in a model of amyotrophic lateral sclerosis caused by expression of a distinct cytosolic misfolded protein, mutant superoxide dismutase (SOD).
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