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The capsids of most plant viruses are simple and robust structures and can be produced in large quantities either by the infection of plants or by expression in a variety of heterologous systems.
No linkage of gpd1 L164P with low glycerol yield was observed in RHA but its effect was revealed by expression in a GPD1-deficient mutant.
Some degradation seems to occur and this could probably be avoided by optimisation of the cell breakage procedure or by expression in a protease deficient strain [ 8].
The mesophilic M. mazei MBF1 was included in this study in order to exclude temperature-dependent effects, which might be caused by expression in a mesophilic host (e.g. incorrect folding, missing activity).
Besides limiting the number of phylogenetically informative nucleotide positions for precise phylogenetic assignment of environmental sequences, obtaining partial sequences also prevents their functional study by expression in a heterologous microbial host.
Together with the fact that overall PSEA more efficiently detected contribution from the more abundant population (i.e. granule cells, see above), we concluded that expression contribution by cell populations of lower abundance could effectively be masked by expression in a more abundant cell population.
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The functional economies envisioned by early functional role semanticists were networks with nodes corresponding to the objects and properties denoted by expressions in a language.
To confirm that the lack of production was due solely to the deletion of cxmK, we attempted to complement the mutant by expression in trans of a wild-type copy of cxmK cloned in the expression vector pIJ10257.
The ability to produce such an agent by expression in E. coli would be advantageous.
Finally, cell death was increased significantly by UbV.7.2 expression in a colon cancer cell line that was treated with the chemotherapy drug cisplatin, demonstrating the therapeutic potential of inhibiting USP7 by this approach.
Transcripts derived from the other promoter in sheep are characterized by imprinted expression in a tissue specific manner.
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