Sentence examples for by expression from a from inspiring English sources

In order to investigate this, a number of site directed mutants were isolated in AapQ and screened for transport specificity by expression from a plasmid in the aap bra null mutant RU1722 (data not shown).

The repressor was engineered by expression from a constitutive yeast promoter, fusion to a viral activator protein cassette, and codon optimization.

To study whether the absence of Brahma is instrumental in the pronounced increase in cell volume, we analysed C33A cells in which Brahma function was restored by expression from a retroviral construct compared with dominant negative mutant of Brahma and empty vector.

Granted, a simple and satisfactory explanation would be that these phenotypes reflect biological activities of dysbindin in neurons that, unlike those in non-neuronal cells, cannot be fulfilled by the protein levels achieved by expression from a single copy of the wild-type allele.

Failure to secrete is unlikely to be caused by expression from a GATEWAY vector, which requires the translation of the attB2 linker (NPAFLYKVV) as shown in Figure 1, because a comparison of a GATEWAY and non-GATEWAY expression vector showed similar levels of expression for Cd200 EC domain fused to rCD4 (domains 3 + 4) (data not shown).

The RLD peptides are fused with a 6×His-tag, by expression from pCold-II vector, allowing their purification with a TALON™ metal affinity resin column as described below.

miRNA levels exhibit a slight drop, but are quickly overtaken by expression from integrated constructs 16 to 20 hours after infection.

Since Wnt2 is a secreted protein, genetic rescue could have occurred by expression from extraneous cell types.

Current developments aim to improve the safety of the replication-competent vectors and to augment the production efficiency of the genome-free vectors by expression from heterologous viral or non-viral vectors.

Cre-mediated recombination is monitored by expression from either the recombination-activatable R26R or R26R-EYFP knock-in alleles [11], [12].

Thus, we expect that the Cbx5 gene has an essential function for viability and that this function is served by expression from both P1 and P2.