Sentence examples for by expression cloning using from inspiring English sources
Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors.
This has been achieved primarily by expression cloning using human autoantibody probes.
Furthermore, by expression cloning using a cDNA library of the human MDR fibrosarcoma cell line HT1080/DR4, we identified the antigen of LMR-12.
NY-ESO-1 antigen, a cancer/testis (CT) antigen, was originally identified in oesophageal cancer by serological expression cloning using autologous patient serum and has been shown to be strongly immunogenic.
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.
The human FRMD7 cDNA, encoding the 714-residue full-length protein, was obtained by PCR amplification from IMAGE clone 40 079 764 and inserted into pLEICS-20 (N-terminal myc tag) and pLEICS-21 (N-terminal GFP tag) mammalian expression vectors by recombination-based cloning, using the University of Leicester Protex cloning service.
MA plots, Pearson correlations, and log fold change plots were made using R. Transcripts identified by differential expression were cloned using forward and reverse primers into a double-stranded RNA expression vector as previously described (Rink et al., 2009).
Gabor et al. (2004) quantified the probability of detection of particular genes by random expression cloning on a theoretical basis using 32 prokaryotic genomes (belonging to Euryarchaeota, Crenarchaeota, Firmicutes, Actinobacteria, and Proteobacteria).
The expression of RBCS1 alleles was also studied by quantitative PCR (qPCR) for the same clones using the expression of the CcUBQ10 gene as an internal reference.
Expression of Bcl-2 was assessed by Western blotting in selected clones using an antibody supplied by Upstate Biotechnology (Lake Placid, NY, USA).
The pcDNA3.1 vector was used to generate JMJD6 expression clones by transfection by using Lipofectamine 2000 (Invitrogen) into MDA-MB231, T47D, and CAMA cell lines.
