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Great strides toward understanding gating in HCNs have been made by expressing and characterizing HCN channels in oocytes or mammalian expression systems for electrophysiological studies.
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An ideal RG should be constitutively expressed and characterized by stable expression in different samples/experimental conditions.
As a control, native MTAN monomers linked by L20 were also expressed and characterized.
The COMM proteins are ubiquitously expressed and characterized by their conserved and distinctive domain (COMM domain) located at the carboxy-terminus.
This R229W variant was recombinantly expressed and characterized by Musayev et al. [47].
Finally, the four consensus candidate mutations, which resulted as the best replacements of the WT sequence, were expressed, and characterized by SPR, CD, and thermal-based shift assay.
In an effort to understand the rapid degradation of GGE by this bacterium, we heterologously expressed and kinetically characterized a suite of dehydrogenase candidates for the first known step of GGE degradation.
Binding DARPins were then expressed, purified and characterized by different ELISAs and cellular assays.
Along these same lines, eggs laid by females expressing genetically characterized spn-E hypomorphic alleles, spn-E, and spn-E (Gillespie and Berg 1995) show a similar phenotype to eggs laid by females from the spn-E allele.
An associated punk subculture emerged, expressing youthful rebellion and characterized by distinctive clothing styles and a variety of anti-authoritarian ideologies.
An associated punk subculture emerged, expressing youthful rebellion and characterized by distinctive styles of clothing and adornment (ranging from deliberately offensive T-shirts, leather jackets, spike bands and other studded or spiked jewelry to bondage and S&M clothes) and a variety of anti-authoritarian ideologies.
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