Thus, a rise in the incidence of ST-32/ET-5 meningococcal disease had possibly been the result of local host populations becoming colonised by meningococci expressing a previously unseen antigenic repertoire followed by a decline in disease several years later once host immunity had increased.
The T cell immunogen, CVB4/p24 733), was constructed previously by expressing a gag p24 sequence as a non-structural peptide at the amino-terminus of the CVB4 polyprotein.
We inhibited TAp63 or TAp73 in T47D and MDA-MB-468 cells by expressing a lentiviral shRNA construct that we previously showed to be a potent and specific inhibitor of this isoform, followed by a brief drug selection (Rocco et al, 2006; Leong et al, 2007).
A similar dilated phenotype has previously been observed by expressing a human cardiac disease mutation in flies [10].
The first problem had previously been addressed by expressing a second heterologous protein as a fusion product with the FMDV 2A protease from modified virus structural proteins [5].
Tip cell sprouting was also inhibited by expressing sFlt1, as previously reported (Zygmunt et al., 2011).
Beneath all that, a dark-age set of attitudes expressed by a previously highly respected young British manager, which were associated by the LMA, after a day's consideration, with "banter".
Wave contributions to ESLs are expressed by a, previously applied, generic approximation of wave setup (refs. 5,62 and M.I.V., manuscript submitted).
DCs were confirmed to express S100A4, a previously reported protein expressed by fibroblasts.
TAT-AS was previously produced by expressing pTAT/pTAT-HA in a BL21 DE3 pLysS E. coli strain [ 5].
