To determine whether expanded expression of has2 caused the increased number of AV endocardial cells we performed Has2 knockdown experiments.
To assess whether expanded expression of has2 caused the myocardial phenotype, we determined the expression of cspg2 and bmp4 after Has2 knockdown in control and npnt morphant embryos.
Consistent with this idea, RNAi knockdown of RpS8 also causes the expanded expression of both Pxn and Hml-gal4 UAS-GFP expression throughout the lymph gland primary lobes.
A similar correlation of expanded expression of CYC-like genes with abortion of both the dorsal and lateral stamens is also observed in Chirita (Gesneriaceae) [ 25].
Taken together, our data show that the increased number of AV endocardial cells in npnt morphants is due to the expanded expression of has2.
Furthermore, fgfr2b knockdown resulted in the expanded expression of roof plate markers and fgfr2b morphants showed very similar dorsal patterning defects to those observed after fgf22 knockdown.
The AfD gives cover to expanded expressions of intolerance and hate.
Huntington disease is characterized by expression of toxic polyglutamine repeat-expanded mutated forms of the protein huntingtin and its aggregation in intranuclear and cytoplasmic inclusions.
Similarly, for both models, we extended previous data (Bolinger et al., 2013, Snyder et al., 2008) and showed overexpression of Klrc1 (NKlrg1, Klre1 (Klra1), Klre1, Klra1 (Ly-49c), and Klrk1 (Nkg2d) in the expanded responses and unique expression of Ly49C mRNA, confirmed by flow cytometry analysis or qPCR (data not shown).
The expression of decorin was low and remained unchanged between cell aggregates formed from FGF2-expanded and non-FGF2-expanded cells, but the expression of decorin was 3-fold to 6-fold lower in cell aggregates than that in tissue.
