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The benefit of our network oriented approach is that it results in a list of subnetworks associated with mammary tumorigenesis, which are formally defined, based on a combination of network connectivity and GO information, and are not constrained by existing annotations of pathways.
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Such topology-based designation of modules is not constrained by existing annotation of pathways, and allows finding novel disease-specific modules.
Of these 79 genes some are described as 'unknown' by existing annotations (Additional file 3: Table S5).
Gene ontology (GO) annotations (http://www.geneontology.org) were obtained by mapping GI numbers of the NCBI non-redundant protein database to the existing annotations of characterized proteins with GORetriever, provided by the AgBase web server (http://www.agbase.msstate.edu).edu
Gene ontology (GO) annotations ([ 54]; http://www.geneontology.org) were obtained from the results of BLASTx analysis by mapping GI numbers of the NCBI-nr protein db to the existing annotations of characterized proteins in the UniProtKB db [ 55].
First, can one perform de novo predictions of phenotypes without relying on existing annotations of genes?
Gene function data were obtained from the existing annotations of the tomato genome.
h Supplementable with 10-fold amounts of biotin compared to the wild-type strain In the majority of clones the observed auxotrophic phenotype is well explained by the existing annotation of the disrupted gene.
However, the existing annotation of the transcriptome was far from being perfect.
In addition to the incomplete annotations, many of the existing annotations are of low quality and may be inaccurate.
Each of the candidate genes was subsequently checked manually by using existing annotations in GenBank to confirm that they encoded the expected NBS proteins.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com