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Finally, differences between the Sanger and 454 data were resolved by examining sequence data from various strains sequenced with Illumina technology.
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DNA sequence alignments for examining sequence data were performed using DNASTAR (DNASTAR Inc. Madison, WI).
In this study, the team examined sequence data from 59 ancient dogs and from more than 2,500 modern canids from around the world.
Heterozygosity was evaluated by examining the sequence data and by forming a heteroduplex on a single individual sample, applying the mismatch endonuclease Cel I, separating products on a denaturing acrylamide gel, and verifying the presence or absence of the predetermined cleaved fragment.
By examining this sequencing data it is possible to get a measure of the methylation state on a per molecule basis by looking at the methylation state of the CpGs on individual reads.
By examining Vtg gene sequence data from other mosquitoes (Cx. pipiens, Ae. aegypti, Ae. polynesiensis, An. gambiae and Oc. triseriatus) we identified T37L homologues upstream of one of the Vtg copies in all species examined.
It does so by examining extant data.
Thus, by examining data from a single Illumina sequencing lane, we can substantially reduce the number of miRNA precursors that generate one expressed mature miRNA.
Traditionally, somatic mutations are detected by examining DNA sequence.
Sex was determined by examining the expression data.
By examining the sequences, they identified 18 kinds of disease-causing bacteria.
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