Moreover, when we examined expression levels by real-time PCR, we could not detect ETRB in the SW1088 cell line after 45 amplification cycles (Fig 4A).
To evaluate the effects of developmental A1254 exposure on specific RyR isoforms, we examined expression levels by Western blot using monoclonal antibodies (mAbs) that selectively bind to RyR1 and RyR3 (mAb 34C) or to RyR2 (mAb C3-33) (Airet et al. 1990; Lai et al. 1992).
To assess whether Dkk4 action in transgenic mice was mediated by a Wnt-Eda cascade, we examined expression levels of the EDA pathway genes Eda, Edar, Shh and LTb [21], [22] in WTDk4TG mice (Fig. 5A).
We examined expression levels of MELK by semi-quantitative RT-PCR and western blot analyses, and found that the MELK-siRNAs si- 3 and si- 3 significandly reduced itsi- 4resi- 4 at the transignificantlynd preducedlevels compared wits scramblexpressionC), si-#1 atd si-#2.
The difference in the gene's transcription levels was confirmed at the protein level by examining expression of p110δ.
We supplemented our immunoblot analysis by examining the expression levels of another detoxifying enzyme, catalase, which revealed substantially decreased levels of catalase in serum-deprived PC12-ND6 cells (results not shown), implying its lack of involvement in NeuroD6-mediated tolerance to oxidative stress and control of ROS production.
We next studied whether increasing the copy number of the PB[BAC] transgene would correlate with elevated of gene expression, by examining the expression level of the RORγ and RORγt transcripts in different PB[BAC] transgenic lines.
Tumors were harvested between 6 and 144 hours later and pharmacodynamic analysis was performed by examining the expression levels of phospho-STAT5, Cdk1 and Hsp70 (Fig. 6B).
The specificity and efficiency of Sp3 siRNA knockdown were confirmed by examining the expression levels of Sp3 and Sp1 by quantitative RT-PCR and western blot (Supplemental Fig. S3).
The specificity and efficiency of Sp1 siRNA silencing were confirmed by examining the expression levels of Sp1 and Sp3 by quantitative RT-PCR and western blot (Supplemental Fig. S3).
