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(3) How are the allele and genotype frequencies of nSNPs modulated by evolutionary variability of amino acid positions?
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The entropy plot revealed the evolutionary variability of nonamer sequences across the WNV proteome (Figure 2).
Further, due to evolutionary variability of the HA protein, development of vaccines against emerging strains is delayed by the non-availability of reagents/antibodies that recognize the new viral strain.
Still, this result could reflect the evolutionary variability of nucleotides at these positions as they show differences between the two pasteurellacean DUESs (Figure 4).
Fierst [ 55] measured the evolutionary variability of genetic systems toward novel phenotypic optima without changing the environmental cue.
Ribosomal DNA (rDNA) is a reliable and informative phylogenetic marker [ 63] that contains sequences with different rates of evolutionary variability.
This might be because evolutionary variability, quantified by a percentage is already size-adjusted, while integral stability is not.
This motivated us to explore the constraints imposed by mRNA secondary structure on evolutionary variability at all codon positions in general, in chloroplast systems.
We found that the evolutionary variability and intrinsic secondary structure stability of these sequences share an inverse relationship.
Calycanthus floridus was the most extreme in this direction, with 40% of its mRNAs showing the positive association between evolutionary variability at a site and stem formation by that site.
This suggests additional evolutionary constraints imposed by the mRNA secondary structure, which motivated us to study its relationship with evolutionary variability.
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