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We assessed the pluripotency of the renewed hESCs both in vitro by evaluation of cellular proliferation, functionality, and gene activities for 21 days, and in vivo by implantation of the stem cell populated scaffolds in an immunodeficient mouse model to induce teratoma formation.
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The method does not compromise skin integrity, as indicated by microscopic evaluation of cellular differentiation, tissue architecture, enzyme activity assessment, dye penetration tests using Franz assay, and cytotoxicity and immunogenicity analyses.
Recent developments in analytical methods allow direct evaluation of cellular physiology by analysis of intracellular variables such as proteins, RNAs or metabolites, either in focused or systems-wide manner [ 1, 3].
Murine and human melanoma cells have been extensively used aiming at the evaluation of cellular and molecular changes induced by toxins from different snake venom metalloproteinases (SVMP).
Collectively, these data indicate that this cell culture model allows for a systematic evaluation of cellular responses that are specifically triggered by levels of intracellular heme exceeding the homeostatic range.
Kaushal, H. et al. Evaluation of cellular immunological responses in mono- and polymorphic clinical forms of post-kala-azar dermal leishmaniasis in India.
Following irradiation, samples were returned to the 37 °C incubator and kept under 5% CO2 for 24 hours before the evaluation of cellular viability with the MTT assay.
18F-3-deoxy-3-fluorothymidine (FLT) is a radiotracer, which allows the evaluation of cellular proliferation.
For research and performance evaluation of cellular networks with femtocells, system-level simulation is one of the most useful methodologies[6].
Nevertheless, the cellular uptake of FLT provides evaluation of cellular proliferation, primarily through reflecting thymidine kinase 1 (TK1) activity.
**Defined by evaluation of parents.
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