The RNA-Seq results were validated by evaluating the levels of expression of selected genes with real-time RT-PCR analyses.
Therefore, the aim of this study was to further characterize mechanisms of resistance in a cohort of previously described S. aureus clinical isolates and in vitro selected mutants with reduced susceptibility to triclosan by sequencing the putative promoter region of fabI and by evaluating the levels of gene expression using microarray analysis.
The possible mechanisms of Gent activities were explored by evaluating expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 using real-time fluorogenic PCR and expression levels of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) using Western blotting.
As such, we confirmed the hepatocyte nature of all the cell lines by evaluating the levels of three hepatocyte-specific transcripts (hepatocyte nuclear factor 4-α, transthyretin, and α-1-antitrypsin) by real-time RTqPCR [18].
In the present study, we address this omission by evaluating current levels of tDDT and PCBs in the blubber of stranded animals.
The recently developed Genomic Allergen Rapid Detection (GARD) assay is an in vitro alternative to animal testing for identification of skin sensitizers, classifying chemicals by evaluating transcriptional levels of a genomic biomarker signature.
By evaluating serum levels of the antibody in patients with different stages of disease, it was found that the concentration of DHCR24 Ab in the HCC cohort was higher than cirrhotic patients, while the levels in cirrhotic patients were higher than the hepatitis cohort and healthy subjects, respectively.
We tested this by evaluating the levels of AMCase and CHI3L1 produced by macrophages isolated from BALF of animals exposed to GO.
Moreover, by evaluating stratified levels of hydroperoxides, we observed markedly elevated frequencies in OC users compared to non-OC users with crude OR = 42, and adjusted OR = 60 for the ≥310 FORT units threshold, and dramatically elevated crude OR = 252, and adjusted OR = 315 considering the ≥400 FORT units threshold.
We carefully normalized tissue lysates for total protein content and controlled our assay by evaluating the levels of several marker proteins, including tubulin (Fig. 1B) and eukaryotic initiation factor 4A (not shown).
