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We analyzed EPO production by evaluating gene expression and confirming it by protein detection.
In the present study, we hypothesized that disease-associated molecular signatures linked to key host response, e.g., airway inflammation and mucus cell metaplasia, could be identified by evaluating gene expression in selected tissues from Scnn1b-Tg mice at critical time points.
Due to the robust morphological activation of macrophages in the Scnn1b-Tg mice, the tendency for genes involved in macrophage function to be up-regulated in whole lung (Additional file 3: Table S1), and the importance of this cell type in lung disease pathogenesis, we continued our studies by evaluating gene expression in purified pulmonary macrophages.
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Indeed, the current data reinforce recent observations made by Roman-Perez and colleagues after evaluating gene expression patterns in 72 breast tissue samples [ 36].
Work by Allinen and coworkers evaluated gene expression profiles of breast cancer stromal cells which were isolated by negatively selecting out epithelial cells, lymphocytes and endothelial cells [ 12].
Cultivars TNG67, TNG72, SCTT and KSWSK, exhibiting diverse degrees of purple color, were selected to evaluate gene expression by real-time PCR.
To determine whether PLD1 gene expression correlates with either the basal or luminal CKs and/or ER status in breast tumour tissue, we isolated RNA from 30 frozen human breast carcinomas and evaluated gene expression by real-time RT PCR.
To validate our phase I findings, we evaluated gene expression by qRT−PCR (TaqMan primer probe assays) on the remaining 28 normal and 30 tumour tissue RNA specimens using a low-density array (LDA).
To evaluate gene expression by the Flow-FISH method we used a linear locked nucleic acid (LNA) probe, GACCACGCAAGAAGCAACAAGAGGG-5′FITC containing nucleic acid analogs with higher affinity for DNA and RNA [ 6].
Primer sequences used for real-time PCR to evaluate gene expression by mesenchymal precursor cells maintained in micromass cultures in the absence and presence of pentosan polysulfate for 7 and 10 days.
To better understand the molecular mechanisms underlying the dynamic alterations in offspring metabolism resulting from an HS maternal diet, we evaluated gene expression by quantitative real-time reverse transcriptase PCR (qRT-PCR).
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