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The possible mechanisms of Gent activities were explored by evaluating expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 using real-time fluorogenic PCR and expression levels of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) using Western blotting.
We also determined whether other hormone genes that are primarily expressed in the pituitary gland were altered in pre-symptomatic scrapie-infected mice by evaluating expression levels of Lhb, Tshb, and Fshb in scrapie-and mock-infected mice.
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We suggest here that microarray experiments using brain-gene expression levels from psychiatric experiments (e.g. schizophrenia group vs non- schizophrenia group) can utilize microarray data not only for group mean effects (i.e. standard microarray analysis) but also, whenever possible, should evaluate expression levels by individual subjects.
In this work, we have performed a preliminary study on the effect of oxygen on the recombinant protein expression by evaluating the expression levels in shake flask cultures with different liquid to flask volume ratios.
This finding was confirmed by evaluating the expression levels of genes expressed during cardiac hypertrophy.
We further examined the inverse correlation between miR-181b and PDCD4 by evaluating PDCD4 expression levels in human CRC cells after miR-181b overexpression or knockdown.
Thus, we explored their potential role in OSCC by evaluating their expression levels in several OSCC cell lines compared with normal human oral keratinocytes.
By evaluating the expression levels of the top 25 strongest sexually dimorphic genes in the individual mice in more detail, substantial variation in expression between the individual pups within the sex groups was observed.
These combined results may imply that, by evaluating the expression level of Nrf2 in the nucleus of gastric cancer cells, it might be possible to predict the best candidates who can benefit from receiving not only adjuvant chemotherapy but also neoadjuvant chemotherapy.
Previous studies evaluating CCR6 expression levels by immunohistology in 64 CRC primary tumor specimens found that CCR6 staining was significantly stronger in cancer cells compared with adjacent colon epithelial cells [24].
In the present study, the effect of CAF on adipocyte differentiation in 3T3-L1 cells was investigated by measuring lipid accumulation and evaluating the expression levels of adipocyte marker genes and their target genes.
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