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The capacity of 5-FU to induce cell death is abrogated by co-treatment with KGF, whereas in KGFR silenced cells 5-FU efficiently induces cell death even combined to KGF, as determined by evaluating cell viability.
The functional status of intact mitochondria was assessed by evaluating cell viability using MTT [ 18].
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Cortical and cerebellar neurons were treated with or without MG132 for 8 hours (long enough to induce accumulation of SP-PrP in cortical cells), then incubated for 16 hours with or without staurosporine, before evaluating cell viability by MTT assay.
Membrane integrity stains aid in evaluating cell viability by inferring that cells with disrupted membranes are dead [ 8] while those with intact membranes have at least retained the possibility of being intact and viable.
The remaining cell solutions in cultured 96-well plate were used to evaluate cell viability by 3- 4,5-dimethylthiazole-2-yl -2,5-diphenyl tetrazolium bromide (MTT) assay.
We incubated cells with the selected aptamers and then evaluated cell viability by the MTT method.
The surface modifications were adopted to evaluate cell viability by BrdU cell proliferation assay.
This was followed by an image analysis to evaluate cell viability [ 9].
Cao et al. [ 104] transfected mouse embryonic stem cells with a fusion reporter gene (RFP, luciferase, and HSV1-tk), evaluated cell viability noninvasively by BLI and PET imaging, and proved the effectiveness of suicide gene therapy.
We evaluated cell's viability by performing cell viability assay in a CellTiter-Glo®luminescent instrument.
The sensitizing effect of DEAB was assessed by sequential 100 μM DEAB and cisplatin treatments and were evaluated by cell viability and sphere-forming efficiency.
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