By establishing protocols for interference with disease-induced miRNA dysregulation in peripheral sensory neurons in vivo, we functionally validate six dysregulated miRNAs as significant modulators of tumour-associated hypersensitivity.
The etiologies of chronic cough were identified and treated by our established protocol [ 15].
In addition, the time taken by each subject to complete the test was recorded manually with a digital stopwatch as provided by the established protocol [ 10, 23– 25].
Samples were prepared for quantification by an established protocol of protein precipitation, collection of supernatants, evaporation, and resuspension into mobile phase before analysis [ 22].
For example, treatment response was evaluated at the physician's discretion rather than by an established protocol, so the imaging modality (CT vs. MRI) and the time of acquisition of imaging studies varied among the enrolled patients.
For the puromycin selection of stable knockdown cells, lentiviruses encoding specific shRNAs were produced by following the established protocol provided by the National RNAi Core Facility, Academia Sinica (Taipei, Taiwan) [ 30].
By following an established protocol, we monitored the pulmonary metastatic potential of tumour cells by living image system at 0, 24 and 48 h after intravenous inoculation.
Plasma cortisol levels were determined by radioimmunoassay following established protocol [13].
The exNO concentration was analyzed with a TE 42S NOx analyzer (Thermo Environment, Franklin, MA, USA) by using an established protocol [ 10].
MPO, indicating pulmonary neutrophil infiltration, was measured in equal-sized samples of the right lungs by using an established protocol [ 21].
Neurite outgrowth started to be visible at the third day of differentiation and after 5 day cells created a highly interconnected and multi-branched "neuronal net" as described by a previously established protocol (Scholz et al. 2011).
