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All dyes presented in this work were obtained by established procedures, as summarized in Figure 2. A detailed description is available in the Supporting Information.
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SDS-PAGE and Western blotting were performed by established procedures.
Thin sections were cut and immunogold labelled by established procedures (Thorpe et al., 1999; Thorpe et al., 2008).
The arcA deletion mutant, designated as JZ3988K (ΔarcA), was constructed using the plasmid pDS3.1 following our established procedure as described in Methods.
However, improved guidance is needed even for established procedures such as ultrasound-guided prostate implants.
Proximate analysis was by ASTM established procedures which covered hemicellulose, cellulose, lignin, moisture, ash and volatile organic matter contents.
Infection of human MSCs in 6 well plates was performed twice by the established procedures [17], [19], [29].
DNA constructions were carried out by using established procedures [31] or following the instructions provided with specific reagents.
Moreover, well-intended established procedures such as fluid resuscitation may impair coagulation homeostasis, thus possibly complicating outcome.
For instance, if nonradioactive PK14105 is injected during brain radiotherapy, the concurrent binding of this drug to the brain cortices may be visualized by subsequent PET imaging using C-PK11195 according to established procedures in humans as by Junck et al. [ 10].
Orange22, founded by Dario Antonioni, a 31-year-old furniture and interiors designer, carries pieces by established designers as well.
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