Sentence examples for by epifluorescence microscopy using from inspiring English sources

After deparafination and rehydration, the slides were stained with 4,6-diamidino-2-phenylindole (DAPI) solution, mounted in PBS, and analyzed by epifluorescence microscopy using a DAPI/FITC/Texas red triple band filter set (Carl Zeiss, Oberkochen, Germany).

Stained cells were documented by epifluorescence microscopy using a Zeiss Axiovert 200 M epifluorescence microscope and the Axio vision software package (release 4.6.3, Zeiss, Jena, Germany).

After 10 min, the samples were wet-mounted on slides and observed by epifluorescence microscopy using an Olympus WIG filter (excitation 380 385 nm; emission >580 nm).

All specimens were imaged by epifluorescence microscopy, using standard dichroic filter sets (Chroma) and a 60×, numerical aperture 1.4 objective (Nikon).

After collection of viral concentrates from the CsCl gradient, the presence of virus-like particles (VLPs) and the absence of microbial contamination were verified by epifluorescence microscopy using SYBR® Gold (Invitrogen: Eugene, Oregon) as described in [56].

The cells were observed by epifluorescence microscopy using an Axiovert 135 TV microscope (Zeiss, Gottingen, Germany).

Coverslips were analyzed by conventional epifluorescence microscopy using a Zeiss Axio imager microscope.

Nuclei were visualized by UV epifluorescence microscopy using a Leica DMI 6000B inverted microscope.

Finally, cells were washed three times in PBS, mounted in DAKO mountain medium and examined by conventional epifluorescence microscopy using a Zeiss Axio imager microscope.

Routinely, polarizing zygotes (4 8 h AF) grown on coverslips were labeled with 5 μM FM4-64 (Molecular Probes Inc., Eugene OR; stock = 20 mM in DMSO) for 30 min and observed by conventional epifluorescence microscopy using a 577.5 632.5 nm band pass emission filter (Chroma Technologies, Brattleboro, VT).

Immunostained sections and cells were examined by phase-contrast and epifluorescence microscopy using an Olympus i X71 microscope (Olympus, Tokyo, Japan).