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Wax esters synthesized by enzymatic transesterification, using palm stearin as raw material, can be considered as an alternative to natural ones.
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To illustrate the proposed framework, assessment on biodiesel production pathway based on enzymatic transesterification using waste oil is conducted.
Recently, enzymatic transesterification using lipase has become more attractive for biodiesel fuel production, since the glycerol produced as a by-product can easily be recovered and the purification of fatty methyl esters is simple to accomplish.
Yields are higher than those obtained by enzymatic transesterification of oil and competitive with the esterification of free fatty acids involving the use of homogeneous acids generating significant amounts of wastewaters.
Sellami et al. investigated the production of a trans-free fat, for margarine formulation, by enzymatic transesterification of palm stearin and palm olein blends.
Nowadays, biodiesel is produced from vegetable oils or animal fats by transesterification using homogenous catalytic processes.
This work reports the production of fatty acid ethyl esters (FAEE) by means of Macauba (Acrocomia aculeata) coconut oil (MCO) solvent-free enzymatic transesterification reactions using a commercial immobilized lipase (Novozym 435) under the influence of ultrasound irradiation.
The enzyme could catalyse the pNPP transesterification using anhydrous methanol and ethanol as the acyl donor group, with 23.60 ± 0.93 and 30.336 ± 2.60 (U/mL) of enzymatic activity, respectively.
The yield from in situ transesterification using an acidic catalyst appeared to be higher than other reported data obtained using the conventional Taguchi method by 27.9~42.6% [ 40– 40].
This process is mainly accomplished by enzymatic methods using cellulases.
The assay was done by enzymatic method using Randox Kit.
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